| Autor: |
Lin, F K, Suggs, S, Lin, C H, Browne, J K, Smalling, R, Egrie, J C, Chen, K K, Fox, G M, Martin, F, Stabinsky, Z |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; November 1985, Vol. 82 Issue: 22 p7580-7584, 5p |
| Abstrakt: |
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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