| Autor: |
Woodward, J G, Orn, A, Harmon, R C, Goodenow, R S, Hood, L, Frelinger, J A |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; June 1982, Vol. 79 Issue: 11 p3613-3617, 5p |
| Abstrakt: |
Mouse L cells transfected with a genomic clone containing the H-2Ld gene (8-5 cells) were shown to function as targets for H-2Ld-specific cytotoxic T lymphocytes (CTL). The CTL-mediated lysis of 8-5 cells was shown to be H-2Ld specific by the use of (i) CTL with restricted reactivity, (ii) unlabeled target inhibiton, and (iii) monoclonal antibody inhibiton. We also demonstrated that 8-5 cells could function as targets for antibody-plus-complement-mediated cell lysis. Specificity was confirmed by using H-2Ld-specific monoclonal antibodies. These experiments demonstrate that the gene products of a major histocompatibility complex genomic clone can be functionally expressed in a foreign cell and can mediate immunologically specific cellular interactions. |
| Databáze: |
Supplemental Index |
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