| Autor: |
McKeehan, W L, Hamilton, W G, Ham, R G |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; June 1976, Vol. 73 Issue: 6 p2023-2027, 5p |
| Abstrakt: |
The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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