| Autor: |
Irving, R A, Toneguzzo, F, Rhee, S H, Hofmann, T, Ghosh, H P |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; February 1979, Vol. 76 Issue: 2 p570-574, 5p |
| Abstrakt: |
Translation of mRNA encoding vesicular stomatitis virus envelope glycoprotein G by as membrane-free ribosomal extract obtained from HeLa cells yielded a nonglycosylated protein (G1 (Mr 63,000). In the presence of added microsomal membranes, G1 was converted to the glycosylated protein (G2 (Mr 67,000) which is inserted in the membrane vesicles as a transmembrane protein. Labeling with methionine donated by wheat germ initiator tRNA1Met showed that G1 but not G2 contains methionine in the NH2-terminal position. Determination of the NH2-terminal sequence of G1, G2, and G showed that a leader peptide of 16 amino acids is present in G1 but absent from the glycosylated proteins G2 and G. This leader peptide contains at least 62% hydrophobic amino acids and is removed presumably during insertion of G1 into the membrane. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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