| Autor: |
Doetsch, P W, Suhadolnik, R J, Sawada, Y, Mosca, J D, Flick, M B, Reichenbach, N L, Dang, A Q, Wu, J M, Charubala, R, Pfleiderer, W, Henderson, E E |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; November 1981, Vol. 78 Issue: 11 p6699-6703, 5p |
| Abstrakt: |
The 3'-deoxyadenosine (cordycepin) analog of (2'-5')oligo(A) [(2'-5')oligoadenylate with a triphosphate at the 5' end], synthesized enzymatically from cordycepin 5'-triphosphate in lysed rabbit reticulocytes or L-cell extracts was (i) inhibitory to translation in lysed rabbit reticulocytes and (ii) metabolically stable in extracts of either L cells or C85-5C lymphoblasts. The 5' dephosphorylated (core) (2'-5')oligo(A) and the core cordycepin analog can replace human fibroblast interferon in preventing the transformation of human lymphocytes after infection with Epstein--Barr virus B95-8 (EBV) as determined by the decreased incorporation of [3H]thymidine into cellular DNA and the inhibition of morphological transformation of EBV-infected lymphocytes. Whereas the naturally occurring core (2'-5')oligo(A) was cytotoxic to uninfected lymphocytes and proliferating lymphoblasts, the core cordycepin analog was not. Human leukocyte interferon was more effective than human fibroblast interferon in the inhibition of EBV-induced transformation of human umbilical cord lymphocytes and adult peripheral blood lymphocytes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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