| Autor: |
Trevor, K, Linney, E, Oshima, R G |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; February 1987, Vol. 84 Issue: 4 p1040-1044, 5p |
| Abstrakt: |
Antisense endo B cytokeratin RNA encoded by a retrovirus vector was expressed in a derivative of the F9 embryonal carcinoma cell line. Two G418-resistant clones were selected that expressed a colinear transcript containing both neomycin and antisense endo B cytokeratin sequences. Expression of a 5-fold excess of antisense endo B RNA over endogenous, retinoic acid-induced endo B RNA resulted in suppression of endo B cytokeratin protein expression. In addition, the normal induction of endo A protein, the type II cytokeratin that polymerizes with endo B, was suppressed at the RNA and protein levels. Revertant clones, which synthesize little if any neo or antisense endo B RNA, regain the ability to express the affected gene products in response to retinoic acid. These results indicate that the suppression of endo B cytokeratin protein synthesis influences the stable levels of endo A mRNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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