| Autor: |
Regan, J W, Kobilka, T S, Yang-Feng, T L, Caron, M G, Lefkowitz, R J, Kobilka, B K |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; September 1988, Vol. 85 Issue: 17 p6301-6305, 5p |
| Abstrakt: |
An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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