Molecular Species Analysis of Polyunsaturated Fish Triacylglycerol by High-Performance Liquid Chromatography/Fast Atom Bombardment Mass Spectrometry

Autor: Hori, Masanori, Sahashi, Yuko, Koike, Seiji, Yamaoka, Ryohei, Sato, Masanori
Zdroj: Analytical Sciences; October 1994, Vol. 10 Issue: 5 p719-724, 6p
Abstrakt: A molecular species analysis of polyunsaturated fatty acid-enriched fish oil, a complex and labile mixture of various triacylglycerols, was carried out by high-performance liquid chromatography/fast atom bombardment mass spectrometry (HPLC/FAB-MS)-with a frit-FAB interface. Positive and negative ion FAB mass spectra and mass chromatograms provided indications of the possible combination of fatty acids in each triacylglycerol component. The actual triacylglycerol retention time values obtained by mass spectrometry were compared with those predicted by the relative retention potential index theory. The two values coincided well with each other. This method thus facilitates the identification of components overlapping in HPLC without fractionation.
Databáze: Supplemental Index