| Autor: |
Sasamoto, Kazumi, Ohkura, Yosuke |
| Zdroj: |
Analytical Sciences; August 1990, Vol. 6 Issue: 4 p505-509, 5p |
| Abstrakt: |
Compounds in which N-acetyl-d-glucosaminide groups were covalently attached to chromogenic metal chelators at their metal-binding sites were synthesized as substrates for N-acetyl-β-d-glucosaminidase (NAGase). Upon incubation with NAGase in the presence of Fe(III), Cu(II) or Co(II) at pH 4.5–5.1, these substrates gave an absorbance increase corresponding to respective metal complexes of their aglycons. Although this approach permitted the rate-assay of NAGase under the conditions of weakly acidic pH (which had not yet been reported), problems using the chelator-conjugated substrates are tend to be hydrolytic instabilities of the substrates and inhibitory effect of the metal ions over the enzyme. |
| Databáze: |
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| Externí odkaz: |
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