| Autor: |
Nakashima, Kenichiro, Maki, Kouichi, Kawaguchi, Shinki, Akiyama, Shuzo, Tsukamoto, Yukie, Imai, Kazuhiro |
| Zdroj: |
Analytical Sciences; October 1991, Vol. 7 Issue: 5 p709-713, 5p |
| Abstrakt: |
Peroxyoxalate chemiluminescence (CL) assay of hydrogen peroxide (H2O2) or glucose was developed by using 2,4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine as a fluorescent component and bis(2,4,6-trichlorophenyl)oxalate (TCPO) as an oxalate. Linear relationships between CL intensity and final concentration of H2O2from 10-8to 10-4M were obtained. The detection limit at the ratio of CL intensities for sample and blank (S/B) of 3 was 10 nM. The precision for five replicate measurements at 10-5and 10-6M of H2O2were 17.6 and 15.7% of relative standard deviations, respectively. α-D-Glucose was transformed to β-D-glucose with mutarotase and converted to H2O2and D-gluconic acid with glucose oxidase, which was detected by using peroxyoxalate CL reaction. A linear calibration graph was obtained up to 1.5×10-4M of glucose solution. The method was applied to the assay of glucose in human serum. The recovery was 98.2% (N=4). The method correlated well with the conventional colorimetric method (r=0.968). |
| Databáze: |
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