| Autor: |
Lavorante, André F., Giné, Maria Fernanda, Gervasio, Ana Paula G., Miranda, Carlos E. S., Fiore, Marli F., Bellato, Cláudia M., Carrilho, Emanuel |
| Zdroj: |
Analytical Sciences; December 2003, Vol. 19 Issue: 12 p1611-1616, 6p |
| Abstrakt: |
A home-made system hyphenating capillary electrophoresis with an inductively coupled plasma mass spectrometer (CE-ICP-MS) for cadmium speciation of protein-binding and free cadmium ions in solution is presented. The CE-ICP-MS interface consisted of an acrylic block with an internal volume ca. 20 µL in which a platinum electrode, a capillary column, and a connection to an ICP nebulizer were inserted. A make-up electrolyte solution containing 50 mmol L-1Tris-HCl buffer solution (pH 9.0) was continuously flowed through the interface to the ICP nebulizer. The separation of free Cd ions, Cd-cysteine, and Cd bounded to metallothionein (MT) isoforms from rabbit liver was carried out by capillary electrophoresis, and the analytes were detected by ICP-MS. The feasibility to isolate metallothionein compounds extracted from the cyanobacterium SynechococcusPCC7942 was demonstrated. The Cd binding proteins were induced in SynechococcusPCC7942 and further analyzed by CE ICP-MS. |
| Databáze: |
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