| Autor: |
B, Vishnu, Ramachandran, Remya, L.V, Aswanilal, A, Gangaprasad, Siril, E.A. |
| Zdroj: |
Ecological Genetics and Genomics; 20220101, Issue: Preprints |
| Abstrakt: |
Garcinia gummi-gutta(Family: Clusiaceae) is a rich source of a natural anti-obesity agent, hydroxy citric acid (HCA). The present approach aimed to analyse the extent of genetic variability and structure of a population of 50 accessions of G. gummi-guttafrom different geographical localities of Kerala state, India using inter simple sequence repeat (ISSR) markers. Among 30 primers tested, 21 showed clear, reproducible and scorable banding patterns. A total of 157 ISSR fragments were observed with an average of 7.48 bands for each primer. An average of 59.52% polymorphic bands were observed among the accessions, with the highest percentage was observed for UBC 809 (87.5%). Polymorphism information content (PIC) value of 0.355 indicating high degree of allele divergence within the individuals. The highest values for Simpson (0.99) and Shannon indices (1.02) substantiated the significant level of polymorphism within the population. Dendrogram with bootstrapping values (100) based on the neighbour joining method distributed the accessions into three main clusters. Relationships exhibited by the accessions discloses continuous geneflow happening between them irrespective of their geographical distances. STRUCTURE analysis revealed the probability of eight subpopulations based on Bayesian clustering. So, ISSR markers can be used as a powerful tool to differentiate closely related individuals. |
| Databáze: |
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