| Autor: |
Delsol, Anne A., Sunderland, Julie, Woodward, Martin J., Pumbwe, Lillian, Piddock, Laura J. V., Roe, John M. |
| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); May, 2004, Vol. 53 Issue: 5 p872-872, 1p |
| Abstrakt: |
<it>Objective</it>: The effect of a single 5 day enrofloxacin treatment on the native <it>Campylobacter</it> <it>coli</it> population in conventionally weaned 5-week-old pigs was investigated. <it>Materials</it>: Twelve pigs were split into two groups of six: one group was treated with a therapeutic dose (15 mg/pig/day) of enrofloxacin and the other remained untreated to act as the control. <it>Campylobacter</it> <it>coli</it> were isolated from faecal samples and tested for ciprofloxacin resistance by measuring MIC values. Mutations in the quinolone resistance-determining region (QRDR) of the <it>gyrA</it> gene of resistant isolates were identified by sequencing and denaturing HPLC. Levels of enrofloxacin and its primary metabolite ciprofloxacin in the pig faeces were also measured by HPLC. <it>Results</it>: No quinolone-resistant <it>C. coli</it> (<it>n</it> = 867) were detected in any of the pigs prior to treatment, indicating <0.1% resistance in the group. Resistant <it>C. coli</it> were isolated from pigs for up to 35 days after treatment with a therapeutic dose. These resistant <it>C. coli</it> had MIC values of 128 mg/L and 8–16 mg/L for nalidixic acid and ciprofloxacin, respectively, and the same single point mutation causing a Thr-86 to Ile substitution in the QRDR was identified in each. The concentration of enrofloxacin in the pig faeces was 2–4 µg/g faeces for the duration of the 5 day therapeutic treatment and was detected up to 10 days post-treatment. Ciprofloxacin was also measured and peaked at 0.6 µg/g faeces in the treated group. <it>Conclusion</it>: This study provides evidence that a single course of enrofloxacin treatment contributes directly to the emergence and persistence of fluoroquinolone resistance in <it>C. coli</it>. |
| Databáze: |
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