Integration of Bdellovibriospp. with SODIS and Moringa oleiferaflocculation to target multi-drug resistant Klebsiella pneumoniaeand Pseudomonas aeruginosa

Autor: Kode, Megan, Waso-Reyneke, Monique, Reyneke, Brandon, Denissen, Julia, Clements-Decker, Tanya, Havenga, Benjamin, Khan, Sehaam, Khan, Wesaal
Zdroj: Journal of Environmental Chemical Engineering; 20220101, Issue: Preprints
Abstrakt: Combination treatments using Bdellovibrio bacteriovorusPF13 and/or TWPF pre-treatment; solar disinfection (SODIS); and Moringa oleiferaflocculation, were investigated for the reduction of antibiotic resistant Klebsiella pneumoniaeand Pseudomonas aeruginosa. After the various stages of treatment, culture- and molecular-based methods were employed to quantify the respective bacteria. Results indicated that pre-treatment with the B. bacteriovorusstrains did not reduce the P. aeruginosacell concentration. The subsequent exposure of the pre-treated and non-pre-treated samples to SODIS (6h) then significantly reduced the P. aeruginosacell counts and gene copies (EMA-qPCR) by up to 7.50 logs, and 4.37 logs, respectively. In contrast, pre-treatment using both B. bacteriovorusstrains (PF13 and TWPF) in combination with SODIS significantly reduced the K. pneumoniaecell counts (8.46 logs) and gene copies (4.40 logs) to below detection limit. While significant log reductions were obtained for the K. pneumoniaesamples pre-treated with B. bacteriovorusPF13 or TWPF, and SODIS only (no pre-treatment), K. pneumoniaestill persisted. Furthermore, flocculation treatment following SODIS did not significantly reduce the concentration of K. pneumoniaeor P. aeruginosa. The antibiogram of the target organisms was compared before and after each treatment stage using the VITEK® 2 Compact System. No difference in the organisms’ susceptibility to the tested antibiotics was observed, with both K. pneumoniaeand P. aeruginosamaintaining their MDR and XDR status, respectively. It is thus recommended that the interaction and predation kinetics of employing multiple predatory bacteria as a pre-treatment to SODIS is explored, as these treatments eradicated the MDR K. pneumoniae.
Databáze: Supplemental Index
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