| Autor: |
Mendelev, Natalia, Zamojski, Michel, Amper, Mary Anne S., Cheng, Wan Sze, Pincas, Hanna, Nair, Venugopalan D., Zaslavsky, Elena, Sealfon, Stuart C., Ruf-Zamojski, Frederique |
| Zdroj: |
STAR Protocols; 20220101, Issue: Preprints |
| Abstrakt: |
Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived post-mortemhuman pituitaries, which is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that: i) it is not limited to fresh tissue, ii) it avoids tissue dissociation-induced transcriptional changes, iii) it includes a novel, automated quality control pipeline. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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