| Autor: |
Pei-hong, WANG, Sai, WANG, Wen-han, NIE, Yan, WU, AHMAD, Iftikhar, YIMING, Ayizekeranmu, Jin, HUANG, Gong-you, CHEN, Bo, ZHU |
| Zdroj: |
Journal of Integrative Agriculture; June 2022, Vol. 21 Issue: 6 p1673-1682, 10p |
| Abstrakt: |
Horizontal gene transfer (HGT) has been well documented as a driving force in the evolution of bacteria. It has been shown that a horizontally acquired gene, xoc_2868, involved in the global response against oxidative stress and pathogenicity of Xanthomonas oryzaepv. oryzicolastrain BLS256. However, as a transcriptional factor (TF), the regulatory mechanism of XOC_2868 has not yet been revealed. Here, evolutionary analysis suggested XOC_2868 might be co-transferred with its physically proximate downstream genes from a Burkholderiaceaeancestor. Interestingly, RNA-seq data of wild-type (BLS256) and Δxoc_2868strains under oxidative stress showed that XOC_2868 did not regulate the expression of its adjacent genes, but remarkably influenced the expression of several genes involved in the extracellular polysaccharide (EPS) production and xanthan biosynthesis. Chromatin immunoprecipitation-sequence (ChIP-seq) combined with transcriptome analysis revealed that XOC_2868 directly regulates a cydABoperon, encoding two subunits of cytochrome bdoxidase and involved in redox balance. Consistent with Δxoc_2868strain, cydA-and cydAB-knockout mutants also showed a higher sensitivity to H2O2along with a reduced bacterial virulence compared with the wild-type strain. Overall, our findings raise the possibility of regulatory circuit evolution shaped by HGT and driven by selection and reveal a novel regulatory pathway that regulates the expression of cytochrome bdoxidase and thus contributes to the virulence of BLS256. |
| Databáze: |
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