Proof‑of‑concept study to quantify changes in intestinal loads of KPC-producing Klebsiella pneumoniaein colonised patients following selective digestive decontamination with oral gentamicin

Autor: Pérez-Nadales, Elena, Natera, Alejandra M., Recio-Rufíán, Manuel, Guzmán-Puche, Julia, Cano, Ángela, Frutos-Adame, Azahara, Castón, Juan José, Elías-López, Cristina, Romero-Saldaña, Manuel, López-Cerero, Lorena, Martínez-Martínez, Luis, Torre-Cisneros, Julián
Zdroj: Journal of Global Antimicrobial Resistance; September 2022, Vol. 30 Issue: 1 p16-22, 7p
Abstrakt: •We developed and validated a qPCR method for quantitative determination of the extent of intestinal colonisation by KPC-producing Klebsiella pneumoniae(KPC-Kp).•This validated qPCR method detects 17% more positives for KPC-Kp and reduces ≥24 h the time required by a reference method based on standard culture.•A rapid and persistent reduction of KPC-Kp intestinal load is observed in about 60% of patients within a month of selective digestive decolonisation with oral gentamicin; however, only 17% of patients reach persitent eradication at this time point.•The death rate was significantly higher among patients with high baseline KPC-Kp intestinal loads.•The validated qPCR method will help to strengthen public health efforts to monitor colonisation by carbapenemase-producing Klebsiella pneumoniaethrough rapid screening of KPC-producing Klebsiella spp.
Databáze: Supplemental Index
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