| Autor: |
Choi, Ji Sun, Kim, Sang Gon, Lahousse, Mieke, Park, Hye-Yeon, Park, Hae-Chul, Jeong, Byeongmoon, Kim, Jinheung, Kim, Sung-Kun, Yoon, Moon-Young |
| Zdroj: |
SLAS Discovery: Advancing Life Sciences R&D; February 2011, Vol. 16 Issue: 2 p266-271, 6p |
| Abstrakt: |
The protective antigen (PA) of Bacillus anthracisis a secreted protein that functions as a critical virulence factor. Protective antigen has been selected as a biomarker in detecting bacterial infection. The in vitro selection method, systematic evolution of ligands by exponential enrichment (SELEX), was used to find single-stranded DNAs that were tightly bound to PA. After 8 rounds of the SELEX process with PA, 4 different oligonucleotides (referred to as aptamers) that contain a 30-residue ssDNA sequence were identified. Dissociation constant (Kd) values with Cy3-attached aptamers were determined via fluorophotometry to be within a nanomolar range. The authors attempted to visualize the detection of PA using an aptamer-based enzyme-linked immunosorbent assay method, which has proven to be successful within a nanomolar Kdvalue range. Furthermore, 2 of the 4 aptamers exhibited specificity to PA against bovine serum albumin and bovine serum. The results of this study demonstrate the analytical potential of an oligonucleotide-based biosensor for a wide variety of applications, particularly in diagnosing disease through specific protein biomarkers. |
| Databáze: |
Supplemental Index |
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