| Autor: |
Santos, Ivonaldo Reis, Ribeiro, Daiane Gonzaga, Távora, Fabiano Touzdjian Pinheiro Kohlrausch, Maximiano, Mariana Rocha, Rabelo, Ana Carolina, Rios, Thuanny Borba, Reis Junior, Fábio Bueno, Megías, Manuel, Silva, Luciano Paulino, Mehta, Angela |
| Zdroj: |
Brazilian Journal of Microbiology; June 2022, Vol. 53 Issue: 2 p595-604, 10p |
| Abstrakt: |
To verify the potential of metabolites extracted from Rhizobium tropicito trigger the priming of defense responses in cruciferous plants, we analyzed the expression of defense-related genes by qRT-PCR. Brassica oleraceavar. capitata, susceptible to Xanthomonas campestrispv. campestris, were grown in greenhouse conditions. At 18 days after sowing, plants were inoculated with 1 mL of 1% concentrated metabolites produced by R. tropici(CM-RT) in the root. In a second experiment, leaves were sprayed with 1 mL of a solution containing 1% CM-RT. Aerial and root tissue were collected separately at 0 (non-treated control condition), 24, and 48 h after application, submitted to RNA extraction and gene expression analysis by qRT-PCR. The results showed that, after root treatment with CM-RT, most evaluated genes were upregulated at 24 h after application and downregulated at 48 h after application in roots, while in leaves, genes were downregulated both at 24 and 48 h after application. On the other hand, leaf treatment with CM-RT showed that most evaluated genes in leaves and roots were upregulated at 24 and 48 h after application. These results indicate that the effect of CM-RT applied in roots seems restricted to the applied region and is not sustained, while the application in leaves results in a more systemic response and maintenance of the effect of CM-RT for a longer period. The results obtained in this study emphasize the biotechnological potential of using metabolites of R. tropicias an elicitor of active defense responses in plants. |
| Databáze: |
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