| Autor: |
Rajakumar, P.D., Gowers, G-O.F., Suckling, L., Foster, A., Ellis, T., Kitney, R.I., McClymont, D.W., Freemont, P.S. |
| Zdroj: |
SLAS Technology: Translating Life Sciences Innovation; June 2019, Vol. 24 Issue: 3 p291-297, 7p |
| Abstrakt: |
Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences. Our platform has been designed to quickly iterate around a design cycle of four protein coding sequences per plasmid, with larger numbers possible with multiplexed genome integrations in Saccharomyces cerevisiae. This work highlights how developing scalable new biotechnology applications requires a close integration between software development, liquid handling robotics, and protocol development. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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