| Autor: |
Ghissing, Upashana, Jayanthan, Kavya, Bera, Paramita, Bimolata, Waikhom, Mitra, Adinpunya |
| Zdroj: |
Brazilian Journal of Botany; June 2022, Vol. 45 Issue: 2 p587-597, 11p |
| Abstrakt: |
Jasminum sambac(L.) Aiton is one of the most popular jasmines cultivated widely in the tropics for scented flowers. Though physiology of floral scent emission in J. sambacwas described, the preceding metabolites that are utilized in the floral tissue for constituting scent volatiles remained hitherto, unexplored. Here, we report targeted profiling of scent volatiles internal pool and major primary metabolites that are synthesized in the petals of J. sambacat six different stages of floral maturation. GC–MS analysis revealed the dominance of benzenoid compounds in the volatiles internal pool namely benzyl alcohol, benzyl acetate and benzyl benzoate. This was apparent from differences in the amount of active PAL, BEAT and glucosidase enzymes (measured in terms of their in vitro activities) at six stages of floral maturation. Moreover, GC–MS analyses of nonvolatile metabolites (preceded by derivatization) also revealed differences in the metabolite distribution patterns consistent with energy requisitions for different stages of floral maturation. These include sugars, amino acids, fatty acids and alcohols that constitute the nonvolatile metabolic pool of the flower. Higher concentrations of sucrose, fructose and trehalose during the peak stage of scent emission along with the presence of several crucial organic acids indicated their indispensability for specialized metabolite (volatiles) accumulation. Furthermore, temporal expression analyses by RT-qPCR of a few key genes of the benzenoid and terpenoid pathways along with genes for sugar transport and hydrolysis suggest an apparent coordination among the metabolites that contribute to the rhythmic production of volatiles in J. sambacflowers. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink