| Autor: |
Fragoso, Pedro, Armijo, Alicia, Gómez, Doris, Gómez, Claudio, Bugueño, Marco, Sánchez, Gittith, Venegas, Juan |
| Zdroj: |
Polish Journal of Microbiology; January 2018, Vol. 67 Issue: 1 p19-26, 8p |
| Abstrakt: |
In order to characterize native strains of Bacillus thuringiensisof the Colombian Caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as B. thuringiensiswere isolated from different soils and muds around the city of Valledupar. Using a biological test, five isolates of B. thuringiensisshowed toxic effect against larvae of Aedes aegypti. PCR methods were used to detect cry1, cry2, cry4B, cry10and cyt1genes. Cry1and cry2genes were detected in 35.7% and 32.1% of the 28 isolates analyzed, respectively. Surprisingly, reduced lengths of cry4Bgene segments were detected in 28.6% of B. thuringiensissamples. The presence of cry10or cyt1was not detected in any of the 28 samples of B. thuringiensis, despite the high sensitivity of the assays used. The results show that B. thuringiensissamples from the Colombian Caribbean have atypical characteristics compared to those of Latin America and elsewhere in the world, which is consistent with the idea that the geographic origin of B. thuringiensissamples is associated with their biological and genetic characteristics. |
| Databáze: |
Supplemental Index |
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