Engineering of Cry3Bb1 provides mechanistic insights toward countering western corn rootworm resistance

Autor: Kuwar, Suyog S., Mishra, Ruchir, Banerjee, Rahul, Milligan, Jason, Rydel, Timothy, Du, Zijin, Xie, Zhidong, Ivashuta, Sergey, Kouadio, Jean-Louis, Meyer, Jason M., Bonning, Bryony C.
Zdroj: Current Research in Insect Science; January 2022, Vol. 2 Issue: 1
Abstrakt: •Cry3Bb1 engineering for receptor retargeting to counter WCR resistance described•Phage display screens against BBMV or recombinant insecticidal-protein receptors•77 gut binding peptides selected and engineered into 284 new variants of Cry3Bb1•112 variants were active against susceptible but not resistant WCR•Engineering of Cry3Bb1 Domain II loops 1 and 2 disrupted insecticidal activity•Sites for Cry3Bb1 engineering and implications for Cry3Bb1 resistance discussed
Databáze: Supplemental Index