| Autor: |
Jensh, R. P., Koszalka, T. R., Jensen, M., Biddle, L., Brent, R. L. |
| Zdroj: |
Development; June 1977, Vol. 39 Issue: 1 p9-21, 13p |
| Abstrakt: |
The rat parietal yolk-sac and its adherent epithelial cells were examined at various stages of gestation using an en face technique. Specimens were observed at both the light and electron microscopic level. Diastase pretreatment and PAS-staining were used to determine the presence of glycogen. As early as the 12th day of gestation the cytoplasm of the parietal yolk-sac cells contained numerous ribosomes and mitochondria and a large amount of endoplasmic reticulum. The glycogen content of the epithelial cells increased from the 12th day of gestation and accumulated in large quantities by the 16th day. By the 17th day many cells exhibited variable degrees of degeneration. Cellular elements of degenerating cells appeared to be trapped within Reichert's membrane. Contrary to the reports of other investigators, the present study indicates that the capsular portion of the parietal yolk-sac consisting of Reichert's membrane and its adherent epithelial cells remained intact until at least the 18th day of gestation. Some of the unique characteristics of the parietal yolk-sac provide experimental models to study the effects of environmental factors on (1) the synthesis of basement membranes, (2) the ageing of cells and (3) the correlation of these histologic changes with the functions of the parietal yolk-sac. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
