| Autor: |
Barber, Aeleta N., Geer, Jack C. |
| Zdroj: |
Development; March 1964, Vol. 12 Issue: 1 p1-14, 14p |
| Abstrakt: |
The teratogenic activity of the purified components of trypan blue was studied in pregnant mice. The blue component induced abnormalities similar in type and frequency to those caused by the whole dye, and the water-soluble purple fraction exerted a mild teratogenesis when the concentration was increased to 0 · 3 per cent. The sodium hydroxide-soluble purple and red fractions were without harmful effects. The blue and water-soluble purple fractions are absorbed and retained by the endodermal cells of the visceral yolk-sac from the time they become differentiated until term. The dye granules are associated with proteinaceous materials in apical vacuoles in the cytoplasm of these cells. Prior to the closure of the yolk-sac the dye has direct access to the embryo. Microscopic examination of unstained sections at this stage in development reveals a diffuse staining of the embryonic mass immediately adjacent to the visceral endoderm. The suggestion is offered that cessation of the teratogenic activity of the dye after closure of the yolk-sac is due to its retention and inactivation by the cells of the yolk-sac epithelium. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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