| Autor: |
Thorp, Barry H., Anderson, Irene, Jakowlew, Sonia B. |
| Zdroj: |
Development; April 1992, Vol. 114 Issue: 4 p907-911, 5p |
| Abstrakt: |
The localization of TGF- β1, - β2 and - β3 was studied in the growth plate, epiphysis and metaphysis of the tibiotarsus of three-week-old chicks. The different TGF- β isoforms were localized to hypertrophic chondrocytes, chondroclasts, osteoblasts and osteoclasts using immunohistochemical staining analysis with specific TGF- β antibodies. TGF- βs in osteoclasts and chondroclasts were restricted to those cells located on the respective matrices. TGF-β3 localization was mainly cytoplasmic in the transitional (early hypertrophic) chondrocytes, but nuclear staining was also detected in some proliferating chondrocytes. The cell-specific localization of these TGF- β isoforms supports the hypothesis that TGF- β has a role in the coupling of new bone formation to bone and cartilage matrix resorption during osteochondral development and suggests that TGF- β may be a marker of chondrocyte differentiation. TGF- β localization preceded a marked increase in type II collagen mRNA expression in transitional chondrocytes, suggesting a role for TGF- β in the induction of synthesis of extracellular matrix. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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