| Autor: |
Burns, Elizabeth M., Christopoulou, Lina, Corish, Pete, Tyler-Smith, Chris |
| Zdroj: |
Journal of Cell Science; August 1999, Vol. 112 Issue: 16 p2705-2714, 10p |
| Abstrakt: |
We have measured the mitotic loss rates of mammalian chromosomes in cultured cells. The green fluorescent protein (GFP) gene was incorporated into a non-essential chromosome so that cells containing the chromosome fluoresced green, while those lacking it did not. The proportions of fluorescent and non-fluorescent cells were measured by fluorescence activated cell sorter (FACS) analysis. Loss rates ranged from 0.005% to 0.20% per cell division in mouse LA-9 cells, and from 0.02% to 0.40% in human HeLa cells. The rate of loss was elevated by treatment with aneugens, demonstrating that the system rapidly identifies agents which induce chromosome loss in mammalian cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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