| Autor: |
Kamalati, T., Thirunavukarasu, B., Wallace, A., Holder, N., Brooks, R., Nakamura, T., Stoker, M., Gherardi, E., Buluwela, L. |
| Zdroj: |
Journal of Cell Science; February 1992, Vol. 101 Issue: 2 p323-332, 10p |
| Abstrakt: |
Scatter factor/hepatocyte growth factor (SF/HGF) is a multifunctional cytokine produced by embryonic fibroblasts and other mesenchymal cells that affects the growth and/or the movement of certain epithelia. Here we report that expression of scatter factor activity by MRC 5 cells, a strain of normal human embryonic lung fibroblasts, is greatly reduced as a result of co-culture of these cells with SVK14, an SV40-transformed human keratinocyte cell line. Using a cDNA probe to the beta chain of human HGF, we have found that the fall in SF activity in MRC 5/SVK14 co-cultures is accompanied by the loss of SF/HGF transcripts. As the inhibition of SF activity coincides with the disappearance of SF/HGF transcript, we conclude that inhibition of the SF activity expressed by MRC 5 cells by co-culture with SVK14 involves transcriptional regulation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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