| Abstrakt: |
In this study, we investigated the effects of Nano-SiO2, ultraviolet (UV) irradiation, and hydrogen peroxide on the growth of four marine aquatic pathogens as well as on their polysaccharide, biofilm formation, protein, and DNA. The results indicated that among all concentrations, 100 μg/L Nano-SiO2demonstrated the most inhibition of Edwardsiella tarda, Vibrio anguillarum, and Vibrio parahaemolyticus. Ultraviolet irradiation for 3 min had the most obvious inhibitory effect on E. tarda, but neither inhibitory nor promoting effects on the other three strains. High hydrogen peroxide concentrations had obvious inhibitory effects on all bacterial growth. Moreover, Nano-SiO2had some influence on bacterial polysaccharide properties and some amount of DNA splicing effect, but no effect was noted on proteins. At present, the recognized pathogenic genes of V. parahaemolyticusinclude tdh, trh, and tlh. We thus studied the effect of Nano-SiO2on these genes and then conducted infection experiments. The results showed that Nano-SiO2further reduced infection with V. parahaemolyticusin shrimp. |
