Co-silencing E1and its homologs in an extremely late-maturing soybean cultivar confers super-early maturity and adaptation to high-latitude short-season regions

Autor: Li-feng, LIU, Le, GAO, Li-xin, ZHANG, Yu-peng, CAI, Wen-wen, SONG, Li, CHEN, Shan, YUAN, Ting-ting, WU, Bing-jun, JIANG, Shi, SUN, Cun-xiang, WU, Wen-sheng, HOU, Tian-fu, HAN
Zdroj: Journal of Integrative Agriculture; February 2022, Vol. 21 Issue: 2 p326-335, 10p
Abstrakt: Soybean (Glycine max(L.) Merr.), a typical short-day plant, is sensitive to photoperiod, which limits the geographical range for its cultivation. In the flowering pathway regulated by photoperiod, E1, a flowering inhibitor in soybean, plays the dominant role in flowering time regulation. Two E1homologs, E1-like-a(E1La) and E1-like-b(E1Lb), play overlapping or redundant roles in conjunction with E1. In the present study, E1and E1La/bwere simultaneously silenced viaRNA interference (RNAi) in Zigongdongdou (ZGDD), an extremely late-flowering soybean landrace from southern China. As a result, RNAi lines showed a much earlier-flowering phenotype and obvious photoperiod insensitivity compared with wild-type (WT) plants. In RNAi transgenic plants, the expression levels of flowering inhibitor GmFT4and flowering promoters GmFT2a/GmFT5awere significantly down- and up-regulated, respectively. Further, the maturity group (MG) of the RNAi lines was reduced from WT ZGDD's MG VIII (extremely late-maturity) to MG 000 (super-early maturity), which can even grow in the northernmost village of China located at a latitude of 53.5°N. Our study confirms that E1and E1La/bcan negatively regulate flowering time in soybean. The RNAi lines generated in this study, with early flowering and maturity traits, can serve as valuable materials and a technical foundation for breeding soybeans that are adapted to high-latitude short-season regions.
Databáze: Supplemental Index
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