Abstrakt: |
ABSTRACTDigestive systems in human, animals, and fish are biological reactors and membranes to digest food and extract nutrients. Therefore, static and dynamic models of in-vitrodigestion systems are developed to study e.g. novel food and feed before in-vivostudies. Such models are well developed for human, but not to the same extent for animals and fish. On the other hand, recent advances in aquaculture nutrition have created several potential fish meal replacements, and the assessment of their nutrient digestibility is critical in the application as a fish meal replacement. Using an in-vitromethod, the assessment of an ingredient digestibility could be faster and less expensive compared to using an in-vivoexperiment. An in-vitromethod has been widely used to assess food nutrient digestibility for humans; however, its application for fish is still in the early stages. Both the human and fish as monogastric vertebrates share similar gastrointestinal systems; thus, the concept from the application for humans could be applied for fish. This review aims to improve the in-vitrodigestion protocol for fish by adapting the concept from then study for humans, summarizing the current available in-vitrodigestion model developed for human and fish in-vitrodigestion study, identifying challenges specifically for fish required to be tackled and suggesting an engineering approach to adapt the human in-vitrogastrointestinal model to fish. Protocols to conduct in-vitrodigestion study for fish are then proposed. |