| Autor: |
Csonka, Katalin, Tasi, Zsolt, Vedelek, Viktor, Vágvölgyi, Csaba, Sinka, Rita, Gácser, Attila |
| Zdroj: |
Virulence; December 2021, Vol. 12 Issue: 1 p2571-2582, 12p |
| Abstrakt: |
ABSTRACTCandidainfections are the most prevalent cause of serious human mycoses and are the third most common pathogens isolated from bloodstream infections in hospitalized patients. C. parapsilosisis a member of the non-albicansspp., which have a predilection for causing life-threatening disease in neonates and hospitalized pediatric patients. In this study, we utilized a Drosophila melanogasterinfection model to analyze the immunological responses to C. parapsilosis. Our results demonstrate that the Toll pathway in Drosophilacontrols C. parapsilosisproliferation as the Toll signaling mutant MyD88−/−flies are highly susceptible to C. parapsilosis. We also confirmed that the MyD88−/−fly is a convenient invertebrate animal model to analyze virulence properties of different species and strains from the C. parapsilosis sensu latocomplex as C. orthopsilosis, C. metapsilosisproved to be less virulent than C. parapsilosis sensu strictoand the N-mannan deficient C. parapsilosis och1Δ/Δ strain showed attenuated pathogenicity in this immunodeficient Drosophilabackground. We also found that Persephone protease is not required for detection and activation of Toll pathway during C. parapsilosisinfection. Furthermore, we observed that Drosophilaβ-glucan receptor deficient flies where more sensitive to C. parapsilosiscompared to wild-type flies; however, we could not find a clear dependence on the recognition of this receptor and the cell wall β-glucan exposure-induced host response. These studies establish this D. melanogasterinfection model as an efficient tool in deciphering immune responses to C. parapsilosisas well as for assessing virulence factors produced by this emerging fungal predator. |
| Databáze: |
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