| Abstrakt: |
It is challenging to convert redox metabolites present inside fungi into measurable signals via permeabilization because of the fungi's thick and rigid cell walls. We report that Aspergillus niger(A. niger) can be detected via the electrochemical measurement of redox metabolites excreted by a low level of permeabilization of the cell wall of A. niger, when incubated in tris buffer containing hydrazine. Electrochemical signals of redox metabolites are amplified by electrochemical–chemical redox cycling, in which hydrazine acts as a reductant for rapid redox cycling. The detection method is specific to A. nigeramong the major three fungi causing aspergillosis. The calculated detection limit for A. nigeris ~2 × 102CFU/ml despite the incubation period being only 5 min, indicating that the detection method is highly sensitive and rapid. The detection method does not require a wash step, specific affinity binding, or pretreatment step, indicating that it is a simple method. Aspergillus nigercan be detected (with high sensitivity, rapidness, simplicity, and specificity) using the electrochemical measurement of redox metabolites excreted by a low level of permeabilization of the fungal cell wall and membrane, which is achieved via brief incubation of A. nigerin tris buffer containing hydrazine. The calculated detection limit for A. nigeris ~2 × 102CFU/ml, and the detection method does not require a wash step, specific affinity binding, or pretreatment step. |
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