| Autor: |
Li, Qiaoyun, Hu, Runyu, Guo, Zhenfeng, Wang, Siyu, Gao, Chuang, Jiang, Yumei, Tang, Jianwei, Yin, Guihong |
| Zdroj: |
The Crop Journal; 20210101, Issue: Preprints |
| Abstrakt: |
Black point disease caused by Bipolaris sorokinianais a problem in wheat production worldwide. We aimed to identify major quantitative trait loci (QTL) for resistance to black point and develop molecular markers for marker-assisted selection (MAS). A recombinant inbred line (RIL) population derived from a cross between Wanyuanbai 1 (susceptible) and SN4143 (resistant) was evaluated for black point response at three locations during two years under artificial inoculation with B. sorokiniana, providing data for six environments. Thirty resistant and 30 susceptible RILs were selected to form resistant and susceptible bulks, respectively, that were genotyped by the wheat 660K SNP array; 685 single-nucleotide polymorphisms (SNPs) were identified, among which 385 (56.2%) and 115 (16.8%) were located on chromosomes 4A and 2B, respectively. Bulked segregant RNA-Seq analysis identified candidate regions on chromosomes 4A (4.60 Mb–40.28 Mb) and 5A (1.22 Mb–48.47 Mb). Genetic linkage maps were constructed for chromosomes 2B, 4A, and 5A using 59 polymorphic dCAPS and SSR markers. Finally, two QTL, designated QBB.hau-4Aand QBB.hau-5A, were detected on chromosomes 4A and 5A, respectively. The resistance allele of QBB.hau-4Awas derived from SN4143, and that of QBB.hau-5Acame from Wanyuanbai 1. QBB.hau-4Awith a large and consistent effect (15.1%) is likely to be a new locus for black point resistance. The markers linked to QBB.hau-4Aand QBB.hau-5Ahave potential application in MAS-based breeding. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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