| Autor: |
Yokoi, Ken-ji, Tsutsui, Sosyu, Arakawa, Gen-ya, Takaba, Masakazu, Fujii, Koichi, Kaneko, Satoshi |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; August 2021, Vol. 85 Issue: 8 p1830-1838, 9p |
| Abstrakt: |
Information about the inulosucrase of nonlactic acid bacteria is scarce. We found a gene encoding inulosucrase (inuBK) in the genome of the Gram-positive bacterium Alkalihalobacillus krulwichiaeJCM 11691. The inuBKopen reading frame encoded a protein comprising 456 amino acids. We expressed His-tagged InuBK in culture medium using a Brevibacillussystem. The optimal pH and temperature of purified InuBK were 7.0-9.0 and 50-55 °C, respectively. The findings of high-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy, and high-performance size-exclusion chromatography with multiangle laser light scattering showed that the polysaccharide produced by InuBK was an inulin with a molecular weight of 3806, a polydispersity index (PI) of 1.047, and fructosyl chain lengths with 3-27 degrees of polymerization. The size of InuBK was smaller than commercial inulins, and the PI of the inulin that it produced was lower.Graphical AbstractComparison of the enzymatic synthesis of inulin by InuBK enzyme and plant extracted inulin and the characteristics of each inulin. |
| Databáze: |
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