| Autor: |
WANG, Sai, WANG, Pei-hong, NIE, Wen-han, CUI, Zhou-qi, LI, Hong-yu, WU, Yan, YIMING, Ayizekeranmu, FU, Luo-yi, AHMAD, Iftikhar, CHEN, Gong-you, ZHU, Bo |
| Zdroj: |
Journal of Integrative Agriculture; December 2021, Vol. 20 Issue: 12 p3222-3229, 8p |
| Abstrakt: |
Horizontal gene transfer (HGT) has been proved a major driving force in prokaryotic evolution. However, the molecular functions of these transferred genes in pathogenic bacteria especially plant pathogenic bacteria are still not fully investigated. In this study, the whole-genome in silicoanalysis was performed and found a syringopeptin synthetase (syp) homolog in Burkholderia glumae,which can cause bacterial panicle blight in rice, was predicted to be horizontally transferred from Pseudomonasancestor with solid confidence by phylogenetic analysis. The comprehensive molecular experiments were performed to study the potential role of this gene in B. glumae.Inoculation of rice panicles with the sypmutant resulted in 60% lower disease index compared with the wild type (WT) parent strain, suggesting the requirement of sypfor the full virulence of B. glumae.Chromatography analysis of exudates from B. glumaeshowed suppression of synthesis of metabolites analogous to syringopeptin in the mutants. All these data raise the possibility of HGT phenomenon in shaping the virulence and adaptation of B. glumaeover evolutionary time. |
| Databáze: |
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