| Autor: |
Moreno-Fernandez, Maria E., Damen, Michelle S.M.A., Divanovic, Senad |
| Zdroj: |
STAR Protocols; December 2021, Vol. 2 Issue: 4 |
| Abstrakt: |
Isolation of viable immune cells from human tissues is critical for the characterization of cellular and molecular processes underlying disease pathogenesis. Here, we describe protocols for the isolation of highly viable immune cells from liver wedges and mesenteric white adipose tissue resections from obese persons. Notably, characterization of the isolated single-immune cell suspensions, via utility of basic immunological interrogations and genetic approaches, promises to generate an improved understanding of altered immunological pathways in obese individuals with or without metabolic diseases. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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