| Autor: |
Semancik, J.S., Morris, T.J., Weathers, L.G., Rordorf, B.F., Kearns, D.R. |
| Zdroj: |
Virology; January 1975, Vol. 63 Issue: 1 p160-167, 8p |
| Abstrakt: |
Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMPUMPand GMPCMPratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm(52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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