Autor: |
Cappellini, Monica, Flaceliere, Maud, Saywell, Veronique, Soule, Julien, Blanc, Emilie, Belouin, Fanny, Ortiz, Erika, Canterel-Thouennon, Lucile, Poupeau, Sophie, Tigrett, Sylvia, Vire, Bérengère, Liaud, Pierre, Blairvacq, Mélina, Joubert, Dominique, Prieur, Alexandre |
Zdroj: |
Analytical Methods; 2021, Vol. 13 Issue: 38 p4468-4477, 10p |
Abstrakt: |
hPG80(human circulating progastrin) is produced and released by cancer cells. We recently reported that hPG80is detected in the blood of patients with cancers from different origins, suggesting its potential utility for cancer detection. To accurately measure hPG80in the blood of patients, we developed the DxPG80test, a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This test quantifies hPG80in EDTA plasma samples. The analytical performances of the DxPG80test were evaluated using standard procedures and guidelines specific to ELISA technology. We showed high specificity for hPG80with no cross-reactivity with human glycine-extended gastrin (hG17-Gly), human carboxy-amidated gastrin (hG17-NH2) or the CTFP (C-Terminus Flanking Peptide) and no interference with various endogenous or exogenous compounds. The test is linear between 0 and 50 pM hPG80(native or recombinant). We demonstrated a trueness of measurement, an accuracy and a variability of hPG80quantification with the DxPG80test below the 20% relative errors as recommended in the guidelines. The limit of detection of hPG80and the limit of quantification were calculated as 1 pM and 3.3 pM respectively. In conclusion, these results show the strong analytical performance of the DxPG80test to measure hPG80in blood samples. |
Databáze: |
Supplemental Index |
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