| Autor: |
Queiroz, Paula A, Meneguello, Jean E, Silva, Bruna R, Caleffi-Ferracioli, Katiany R, Scodro, Regiane BL, Cardoso, Rosilene F, Marchiosi, Rogério, Siqueira, Vera LD |
| Zdroj: |
Future Microbiology; October 2021, Vol. 16 Issue: 15 p1195-1207, 13p |
| Abstrakt: |
Aim:To elucidate the changes in protein expression associated with polymyxin resistance in Klebsiella pneumoniae, we profiled a comparative proteomic analysis of polymyxin B-resistant mutants KPC-2-producing K. pneumoniae, and of its susceptible counterparts. Material & methods:Two-dimensional reversed phase nano ultra-performance liquid chromatography mass spectrometry was used for proteomic analysis. Results:Our results showed that the proteomic profile involved several biological processes, and we highlight the downregulation of outer membrane protein A (OmpA) and the upregulation of SlyB outer membrane lipoprotein (conserved protein member of the PhoPQ regulon) and AcrA multidrug efflux pump in polymyxin B-resistant strains. Conclusion:Our results highlight the possible participation of the SlyB, AcrA and OmpA proteins in the determination of polymyxin B heteroresistance in KPC-2-producing K. pneumoniae. |
| Databáze: |
Supplemental Index |
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