Detection of DNA damage by alkaline comet assay in mouse colonic mucosa

Autor: Boutet-Robinet, Elisa, Haykal, Maria M., Hashim, Saleha, Frisan, Teresa, Martin, Océane CB.
Zdroj: STAR Protocols; 20210101, Issue: Preprints
Abstrakt: We recently characterized the association between DNA damage and immunoresponse in vivoin colonic mucosa of mice infected with a SalmonellaTyphimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay, MIRCA (Moller et al., 2020)).
Databáze: Supplemental Index