| Autor: |
Hung, Yu-Chien, Huang, Kuan-Lin, Chen, Po-Lin, Li, Jeng-Lin, Lu, Serena Huei-An, Chang, Jui-Chih, Lin, Han-Yi, Lo, Wen-Chun, Huang, Shu-Yi, Lee, Tai-Ting, Lin, Tai-Yi, Imai, Yuzuru, Hattori, Nobutaka, Liu, Chin-San, Tsai, Su-Yi, Chen, Chun-Hong, Lin, Chin-Hsien, Chan, Chih-Chiang |
| Zdroj: |
Cell Reports; September 2021, Vol. 36 Issue: 12 |
| Abstrakt: |
Human ubiquinol-cytochrome creductase core protein 1 (UQCRC1) is an evolutionarily conserved core subunit of mitochondrial respiratory chain complex III. We recently identified the disease-associated variants of UQCRC1 from patients with familial parkinsonism, but its function remains unclear. Here we investigate the endogenous function of UQCRC1 in the human neuronal cell line and the Drosophilanervous system. Flies with neuronal knockdown of uqcrc1exhibit age-dependent parkinsonism-resembling defects, including dopaminergic neuron reduction and locomotor decline, and are ameliorated by UQCRC1expression. Lethality of uqcrc1-KO is also rescued by neuronally expressing UQCRC1, but not the disease-causing variant, providing a platform to discern the pathogenicity of this mutation. Furthermore, UQCRC1 associates with the apoptosis trigger cytochrome c(cyt-c), and uqcrc1deficiency increases cyt-cin the cytoplasmic fraction and activates the caspase cascade. Depleting cyt-cor expression of the anti-apoptotic p35 ameliorates uqcrc1-mediated neurodegeneration. Our findings identify a role for UQCRC1 in regulating cyt-c-induced apoptosis. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
