| Autor: |
Bloch, D B, Bonventre, J V, Neer, E J, Seidman, J G |
| Zdroj: |
Molecular and Cellular Biology; December 1989, Vol. 9 Issue: 12 p5434-5439, 6p |
| Abstrakt: |
The physiological role of the alpha o subunit of guanine nucleotide-binding (G) protein was investigated with a murine adrenal cell line (Y1) transfected with a rat alpha o cDNA cloned in a retroviral expression vector. The parental cell line lacked detectable alpha o subunit. Expression of the alpha o cDNA in transfected cell lines was confirmed by Western blot (immunoblot) analysis. The rat alpha o subunit interacted with murine beta and gamma subunits and associated with cell membranes. Y1 cells containing large amounts of alpha o subunit had altered cellular morphology and reduced rate of cell division. In addition, GTP-gamma S-stimulated release of arachidonic acid from these cells was significantly increased compared with that in control cells. The alpha o subunit appears directly or indirectly to regulate cellular proliferation, morphology, and phospholipid metabolism. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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