| Autor: |
Alonso, J, Sánchez de Miguel, L, Montón, M, Casado, S, López-Farré, A |
| Zdroj: |
Molecular and Cellular Biology; October 1997, Vol. 17 Issue: 10 p5719-5726, 8p |
| Abstrakt: |
Changes in endothelial nitric oxide synthase (eNOS) expression may be involved in the endothelium-dependent vasorelaxation dysfunction associated with several vascular diseases. In the present work, we demonstrate that eNOS mRNA contains a previously undescribed cis element in the 3' untranslated region (3' UTR). A U+C-rich segment in the 3' UTR is critical in complex formation with bovine aortic endothelial cell cytosolic proteins. Tumor necrosis factor alpha (TNF-alpha), which destabilizes eNOS mRNA, increased the binding activity of the cytosolic proteins in a time-dependent manner. These data suggest that endothelial cytosolic proteins bind to the 3' UTR of eNOS mRNA. These proteins may play a role in TNF-alpha-induced eNOS mRNA destabilization. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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