Development and Evaluation of a Point-of-Care Test in a Low-Resource Setting with High Rates of Chlamydia trachomatisUrogenital Infections in Fiji

Autor: Dean, Deborah, Swaminathan, Sumeetha, Kama, Mike, Goemans, Sophie, Faktaufon, Daniel, Alnabelseya, Noor, Spelke, Dawn, Kahrizi, Kamin, Black, Matthew, Mitra, Debkishore
Zdroj: Journal of Clinical Microbiology; May 2021, Vol. 59 Issue: 7
Abstrakt: Rapid and precise detection of Chlamydia trachomatis, the leading global cause of sexually transmitted infections (STI), at the point of care (POC) is required for treatment decisions to prevent transmission and sequelae, including pelvic inflammatory disease, ectopic pregnancy, tubal factor infertility, and preterm birth. We developed a rapid POC test (POCT), termed LH-POCT, which uses loop-mediated amplification (LAMP) of nucleic acids, and performed a head-to-head comparison with the Cepheid Xpert CT/NG assay using clinician-collected, deidentified paired vaginal samples from a parent study that consecutively enrolled symptomatic and asymptomatic females over 18?years of age from the Ministry of Health and Medical Services Health Centers in Fiji.
Databáze: Supplemental Index