High-Content Screening, a Reliable System for Coxiella burnetiiIsolation from Clinical Samples

Autor: Francis, Rania, Mioulane, Maxime, Le Bideau, Marion, Mati, Marie-Charlotte, Fournier, Pierre-Edouard, Raoult, Didier, Bou Khalil, Jacques Yaacoub, La Scola, Bernard
Zdroj: Journal of Clinical Microbiology; April 2020, Vol. 58 Issue: 5
Abstrakt: Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although current serological and molecular detection tools allow a reliable diagnosis of the disease, culture of C. burnetiistrains is mandatory to assess their susceptibility to antibiotics and sequence their genome in order to optimize patient management and epidemiological studies. However, cultivating this fastidious microorganism is difficult and restricted to reference centers, as it requires biosafety level 3 laboratories and relies on cell culture performed by experienced technicians.
Databáze: Supplemental Index