| Autor: |
Mann, B J, Akins, R A, Lambowitz, A M, Metzenberg, R L |
| Zdroj: |
Molecular and Cellular Biology; March 1988, Vol. 8 Issue: 3 p1376-1379, 4p |
| Abstrakt: |
van+, a gene encoding a phosphorus-repressible phosphate permease, was isolated by its ability to complement nuc-1, a positive regulatory locus that normally regulates van+ expression. This was unexpected because the nuc-1 host already contained a resident van+ gene. Plasmids carrying van+ complemented a nuc-2 mutation as well. Probing of RNA from untransformed wild-type (nuc-1+) and constitutive (nuc-1c) strains by van+ probes indicated that levels of the van+ transcript were subject to control by nuc-1+. Probing of the same RNAs with a cosmid clone, containing approximately 15 kilobases of upstream and downstream DNA, revealed no other detectable phosphorus-regulated transcripts within this 40-kilobase region of the chromosome. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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