| Autor: |
Mercer, R W, Schneider, J W, Savitz, A, Emanuel, J, Benz, E J, Levenson, R |
| Zdroj: |
Molecular and Cellular Biology; November 1986, Vol. 6 Issue: 11 p3884-3890, 7p |
| Abstrakt: |
We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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