Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae

Autor: Valerie, K, Fronko, G, Henderson, E E, de Riel, J K
Zdroj: Molecular and Cellular Biology; October 1986, Vol. 6 Issue: 10 p3559-3562, 4p
Abstrakt: A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance in the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylase activity.
Databáze: Supplemental Index