| Autor: |
Silverman, S J, Rose, M, Botstein, D, Fink, G R |
| Zdroj: |
Molecular and Cellular Biology; October 1982, Vol. 2 Issue: 10 p1212-1219, 8p |
| Abstrakt: |
The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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